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Qu ameliorates intestinal barrier and inflammation in DSS-induced colitis in mice. ( A–C ) The protein expression of claudin-1 and occludin was detected by Western blot. Representative IHC staining images of colonic tissues showing IL-6 ( D ) and IL-1β ( F ) expression (scale bar = 200 µm). Semi-quantitative analysis of IL-6 ( E ) and IL-1β ( G ) based on IHC staining. The relative mRNA expression of IL-6 ( H ), TNF-α ( I ), and IL-1β ( J ) in colon tissue was detected by RT-qPCR. Data were shown as mean ± SEM ( N = 3). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs the DSS group. ns, not significant.

Journal: mSystems

Article Title: Quercetin alleviates ulcerative colitis via regulating gut microbiota and tryptophan metabolism

doi: 10.1128/msystems.00703-25

Figure Lengend Snippet: Qu ameliorates intestinal barrier and inflammation in DSS-induced colitis in mice. ( A–C ) The protein expression of claudin-1 and occludin was detected by Western blot. Representative IHC staining images of colonic tissues showing IL-6 ( D ) and IL-1β ( F ) expression (scale bar = 200 µm). Semi-quantitative analysis of IL-6 ( E ) and IL-1β ( G ) based on IHC staining. The relative mRNA expression of IL-6 ( H ), TNF-α ( I ), and IL-1β ( J ) in colon tissue was detected by RT-qPCR. Data were shown as mean ± SEM ( N = 3). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs the DSS group. ns, not significant.

Article Snippet: After being blocked at room temperature for 1.5 h, four primary antibodies were added successively and incubated at 4°C for 16 h: the tight junction protein Occludin (1:1,000, Proteintech, 66378-1-Ig), Claudin-1 (from the same brand, 1:1,000), Cytochrome P450 1B1 (Proteintech, 18505-1-AP, 1:1,000), and the internal reference GAPDH (Affinity, 13050-1-AP/T0004, 1:10,000).

Techniques: Expressing, Western Blot, Immunohistochemistry, Quantitative RT-PCR

Fecal microbiota transplantation from Qu-treated mice alleviated DSS-induced colitis in mice. ( A ) Flow diagram of the experiment. ( B ) Body weight change (%). ( C ) DAI score. ( D ) Colon image. Histogram statistic of colon length ( E ) and spleen index ( F ). Data were shown as mean ± SEM ( N = 6). ( G ) Representative H&E staining image of colon tissue (scale bar = 200 µm). The relative mRNA expression of IL-6 ( H ) and IL-1β ( I ) in colon tissue was detected by RT-qPCR. ( J–L ) The protein expression of occludin and claudin-1 was detected by Western blot ( N = 3). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs the DSS group. ns, not significant.

Journal: mSystems

Article Title: Quercetin alleviates ulcerative colitis via regulating gut microbiota and tryptophan metabolism

doi: 10.1128/msystems.00703-25

Figure Lengend Snippet: Fecal microbiota transplantation from Qu-treated mice alleviated DSS-induced colitis in mice. ( A ) Flow diagram of the experiment. ( B ) Body weight change (%). ( C ) DAI score. ( D ) Colon image. Histogram statistic of colon length ( E ) and spleen index ( F ). Data were shown as mean ± SEM ( N = 6). ( G ) Representative H&E staining image of colon tissue (scale bar = 200 µm). The relative mRNA expression of IL-6 ( H ) and IL-1β ( I ) in colon tissue was detected by RT-qPCR. ( J–L ) The protein expression of occludin and claudin-1 was detected by Western blot ( N = 3). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs the DSS group. ns, not significant.

Article Snippet: After being blocked at room temperature for 1.5 h, four primary antibodies were added successively and incubated at 4°C for 16 h: the tight junction protein Occludin (1:1,000, Proteintech, 66378-1-Ig), Claudin-1 (from the same brand, 1:1,000), Cytochrome P450 1B1 (Proteintech, 18505-1-AP, 1:1,000), and the internal reference GAPDH (Affinity, 13050-1-AP/T0004, 1:10,000).

Techniques: Transplantation Assay, Staining, Expressing, Quantitative RT-PCR, Western Blot